According to Chargaff's Rule, which base pairs with adenine in DNA?

Chargaff's Rule states that in DNA, the amount of adenine (A) is always equal to the amount of thymine (T). This means that adenine pairs specifically with thymine in the double helical structure of DNA. This complementary base pairing is crucial for the stability of the DNA molecule and explains how genetic information is accurately replicated during cell division.

Cytosine and guanine are also complementary in DNA, forming a pair, but they do not directly pair with adenine. Uracil, while it pairs with adenine in RNA, is not found in DNA and therefore is not relevant in the context of base pairing in DNA. Thus, thymine is the correct base that pairs with adenine, adhering to Chargaff's Rule.